Molecular formula: C12H13N5ABOUT4
CAS# 1191237-69-0
Molecular weight: 291.26
(2R, 3R, 4S, 5R) -2- (4-aminopyrrolo [2,1-f] [1,2,4] triazin-7-yl) -3,4-dihydroxy-5- (hydroxymethyl) tetrahydrofuran- 2-carbonitrile
White powder with slightly yellowish tint
Synonym – EVO984
Mechanism of action
GS-441524 (GS = Gilead Sciences), is nucleoside analog and molecular prodrug to a pharmacologically active nucleoside triphosphate molecule. Thus, GS-441524 becomes active only after intracellular metabolisation to triphosphate, which is a false substrate for viral RNA dependent RNA polymerase. It is therefore inhibitor, which acts as a termination sequence (terminator) of the viral RNAchain. GS441524 triphosphate by its incorporation interrupts transcription process and the virus can no longer replicate.
Preparation of GS-441524 for injection use
During clinical trials, GS-441524 was dissolved to a concentration of 10 or 15 mg / ml in a solution containing:
5% ethanol
30% propylene glycol
45% PEG 400 (polyethylene glycol)
20% water with HCl to adjust the resulting pH to 1,5
The resulting mixture was shaken to dissolve in a sterile 50 ml flask and then placed in an ultrasonic bath for 5-20 minutes until it became completely clear. The drug thus dissolved was then stored in a refrigerator and used within 3-4 weeks.